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CARCINO screen®

  1. CARCINOscreen®

CARCINOscreen®

 

What is CARCINOscreen®?

Short-term carcinogenicity prediction system; CARCINOscreen® offers the predictive information for the carcinogenic potential of chemcials by the gene expression profiles in the liver of rats obtained in short-term animal experiments (14days or 28 days) (Fig. 1) (Matsumoto et al., 2014).

  • Fig.1 Overview of CARCINOscreen
    Fig.1 Overview of CARCINOscreen®

【Applicable Conditions】

Species/Strain/Sex Crlj:CD (SD) rat or F344 rat, male
Organ Liver
Number of Animals
for Gene Expression Analysis
3 animals/group
Dosage Concurrent vehicle control + Chemical treatment groups (more than 2 groups)
Route of Administration gavage
Duration of Treatment 14 days or 28 days
Microarray Platform ・Whole Rat Genome Toxplus (Agilent, Custom Array)
・Rat Genome 230 2.0 Array (GeneChip®; Affymetrix)

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Highlights of CARCINOscreen®

  • Short-term, High-accuracy and Low-cost as carcinogenicity screening
    Accuracy (concordance) is more than 90%.
    Quantitative prediction results can be obtained as shown in Fig. 2.
  • Applicable to both Ames-positive carcinogens and Ames-negative carcinogens (Fig.2)
  • Fig.2 Prediction results by CARCINOscreen (73 chemicals)
    Fig.2 Prediction results by CARCINOscreen® (73 chemicals)
  • Various services
    Depending on your experimental design, we can offer various types of services (strain, treatment duration, microarray platform).
  • Allow you a comprehensive gene expression analysis at the same time

 

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Service Menu for CARCINOscreen®

Table 1 Service menu for Short-term carcinogenicity screening (CARCINOscreen®
Menu# Strain Treatment
 duration
Microarray
 platform
Notes
Menu-(1) F344 rat 28 days Toxplus array*2
(Agilent)
-
Menu-(2) SD rat*1 28 days Applicable to generally used strain for animal toxicity testing
Menu-(3) F344 rat
SD rat*1
14 days GeneChip®
(Affymetrix)
  • Shorter animal testing
  • Use of top-shelf microarray

*1:Crlj:CD (SD) rat
*2:Whole Rat Genome Toxplus (Agilent, custom array)

Menu-(1) Prediction using Toxplus array, applying 28-day treatment sample in F344 rat

This menu is applicable to the liver sample obtained from F344 rat. Whole Rat Genome Array Toxplus (herein after Toxplus array) is used for gene expression analysis. Toxplus array is the specifically designed microarray, carrying prediction genes customized for carcinogenicity prediction by CARCINOscreen® and whole genes (approximately 40,000 genes) on “Whole Rat Genome array (Agilent)”. This array offer you not only the predictive result for carcinogenicity, but also a comprehensive gene expression analysis data, at the same time.

Menu-(2) Prediction using Toxplus array, applying 28-day treatment sample in SD rat

This menu is achieved by expanding the application of [Menu (1)] to SD rat that is generally used for animal toxicity testing. Toxplus array is used for the gene expression analysis. To extend [Menu (1)] application, the system was validated using 13 chemicals that had been tested in F344 rat. The results revealed that all liver carcinogens were detected (9/9 chemicals), and that 8 of them were Ames-negative liver carcinogens (Table 2). Among 13 chemicals, 11 chemicals were correctly predicted and this system is judged to be also applicable to the liver from SD rat.

Table 2 Prediction results of 13 chemicals in SD rat

 

Chemical Name Known information Prediction results by CARCINOscreen®
Carc.* Rat Ames F344 rat SD rat
Liver
Carc.
2,4-Diaminotoluene + + + positive positive
Methyl carbamate + + positive positive
Thioacetamide + + positive positive
Urethane + + positive positive
D,L-Ethionine + + positive positive
Chlorendic acid + + positive positive
Clofibrate + + positive positive
Di(2-ethylhexyl)phthalate
(DEHP)
+ + positive positive
3-(p-Chlorophenyl)-
1,1-dimethylurea
+ + positive positive
Di(2-ethylhexyl)adipate
(DEHA)
+ positive negative
2,6-Diaminotoluene + negative negative
2-Chloromethylpyridine HCl + negative negative
Iodoform + negative positive

*:Carcinogneicity

Menu-(3) Prediction using GeneChip®, applying 14-day treatment sample

This menu is applicable to the test sample obtained from shorter-treatment, i.e., 14-day. GeneChip® is applied for gene expression analysis. This menu allow you the use of liver samples from a preliminary test (e.g., dose setting test) of toxicity testing. If you have already measured the gene expression data by GeneChip®, the data can also be applied to our system.

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Option Services

  • From animal testing and microarray analysis to carcinogenicity prediction by CARCINOscreen®.
  • From microarray experiment to prediction, if you already have liver samples.
  • Carcinogenicity prediction only, if you already have gene expression data (Toxplus array or GeneChip®)
  • Fig.2 Prediction results by CARCINOscreen (73 chemicals)
    Options for placing your order

【Flow for your order】

1) Present our analysis plan We will make plan according to your purpose and experimental system.
2) Prepare cost estimation We will present the cost estimation according to the plan.
3) Receipt your sample or data Please send us your sample (chemical, organ sample (stored in RNA later), or total RNA) or data file depending on your plan.
4) Animal testing/
   microarray experiment/
   carcinogenicity prediction
Experiments are conducted according to your plan.
5) Deriver the outcomes The report for each experiment will be prepared and delivered with all data file obtained.

 

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Deliverables/Delivery Date

In addition to the report (printed matter), we will deliver the following data as an electronic file. The contents of the report includes (summary), purpose, method, results (discussion).

Deliverables

(1) Test report (PDF file)
(2) Image file(TIFF file) 
(3) Output file (including prediction sore etc.) (Excel file)
(4) Gene network diagram (JPEG file)

Delivery Date

Please contact us.
(it differs depending on you order and timing of order)

 

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Prices

Please contact us.

 

Ordering

  • Please provide us your information (purpose of the analysis, experimental conditions etc.) as much as possible using this form. Specific analysis conditions will be determined after consultation with you.
  • We will apply the contracts to provide our service.

 

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History of CARCINOscreen® Development

We, CERI, had developed a carcinogenicity prediction method based on gene expression analysis obtained from microarray in NEDO Toxicogenomics Project in 2001-2006 (lead by Professor T. Shirai (Nagoya City Univercity). The resultant microarray data in the project were registered in GEO (Gene Expression Omnibus) database in NCBI (National Center for Biotechnology Information) (available 3-day treatment data14-day treatment data28-day treatment data). Based on the results from this NEDO project and our additional research, CERI put them into a practical use as a carcinogenicity prediction screening tool, named as CARCINOscreen®, and launched its service in 2010.

 

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Simpler alternative to CARCINOscreen® based on quantitative PCR (qPCR)

For the purpose of more simple prediction, we minimize the prediction genes used in CARCINOscreen® and developed the qPCR based prediction method (Saito et al., 2016) in Tox-Omics project in 2012-2016. This method achieves qualitative carcinogenicity prediction with low cost than CARCINOscreen®. The experimental protocol can be found from here.